Next Generation Sequencing Core
NEXT is a modern NGS core facility, consisting of a Laboratory, which allows high high throughput, low cost, production-scale sequencing experiments, combined with a data-intensive computational infrastructure (8000+ cores HPC Cluster with 4PB storage). An infrastructure, unique in Italy, designed to provides specialized capabilities for tracking all-data procedures and biodata provenance, managing complex analysis workflows and results.
NEXT has recently acquired new instruments, including Illumina's most advanced sequencer, the NovaSeq X Plus.
Services on NovaSeq X Plus
Library preparation + sequencing + bioinformatics analysis + storage
RNA Seq
(~ 100 M Paired-end Reads)
Whole Genome Sequencing Human | Animal | Plant | Microbial
WGS (~ 120 Gb = Coverage 30X)
The Library Preparation protocols can be tailored according to the specific requirements.
Whole Exome Sequencing Human | Animal | Plant | Microbial
WES (~ 15 Gb = Coverage 100X)
v7 (35Mb)
The Library Preparation protocols can be tailored according to the specific requirements.
miRNA Seq
(~ 10 M Single Reads)
More Services
Metagenomics
On MiSeq
coverage ≥ 200X
coverage ≥ 500X
Custom Panels and Protocols
On NovaSeq X Plus | On MiSeq
The Library Preparation protocols for WGS, WES, and Transcriptome can be tailored to meet specific requirements.
High resolution HLA Typing up to the sixth digit
Complete gene sequence for HLA loci (RUO): -A, -B ,-C, -DRB1, -DPB1, -DQB1, -G
For custom panels and specific protocols, please contact us.
Sequencing only
On NovaSeq X Plus - flow cell 10B:
On MiSeq - MiSeq Reagent Kit:
v3 (150/600-cycles); ~23 MCluster; ~3,5/~14 Gb
v2 (300/500-cycles); ~13 MCluster; ~4,5/~8 Gb
v2 Micro (300-cycles); ~4 MCluster; ~1 Gb
v2 Nano (300/500-cycles); ~1 MCluster; ~300/~500 Mb
Tailored services
Lab or Remote
We can offer personalized services, consultancy, and advanced training, both on-site at our laboratory and remotely. Please contact us.
Next Generation Sequencing
Call for partner: The NGS platform is available to the scientific community for research project collaborations
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OUR NGS LABORATORY
Our NGS Lab has been a pioneer in the use of NGS technology, starting from 2009 with the first sequencers produced by Illumina, two Genome Analyzers. By 2010, we upgraded to three HiSeq2000 sequencers, and later to the HiSeq3000. We have 15 years of experience, dozens of different applied protocols, and have processed thousands of samples of various types and origins.
Recent acquisitions have led to a technological upgrade of our laboratory, aimed at increasing the accuracy, reliability and production capacity of sequencing data.
Illumina NovaSeq X Plus the latest and and most powerful sequencer, with a production capacity of 120Tb/month
Agilent Fragment Analyzer 5300, Capillary electrophoresis (48 capillaries) for fragment analysis and quality control of nucleic acids
Tecan Infinite 200 Pro plate reader for fluorometric quantification of nucleic acids and libraries
Illumina MiSeq sequencer for metagenomics, small panels and other custom protocols
Liquid Handler Hamilton NGS STAR Liquid Handler for Library Preparation with throughputs up to 96 sample libraries processed at once
The platform is directly interconnected with the HPC resources of the CRS4 computing center, a unique infrastructure in Italy that enables large-scale sequencing projects.
A web-accessible database will be integrated with a LIMS (Laboratory Information Management System) and analysis pipeline management system. The platform is designed to allow facility users to require analyses, perform customized investigations, and organize, consult, and update data related to their projects.
Areas of interest
Sequencing of the human genome or a set of specific genes for the investigation of a wide range of DNA variations that can be causative or predisposing to disease, or involved in drug response modulation; analysis of the transcriptome (RNASeq) to highlight variations in gene expression; high-resolution HLA gene typing with ambiguity elimination; characterization of the microbiota to identify pathogens; characterization of bacterial and viral pathogen genomes for epidemiological studies or to understand mechanisms of drug resistance and virulence.
Information on the complete genome of multiple animal and plant species enables the selection of more productive and disease-resistant strains and their geographic traceability; analysis of environmental DNA metagenome in agri-food production chains for the detection of contaminating species (viruses, bacteria, fungi and parasites, animals and plants) or identification of pathogens that affect crop or livestock productivity; species authentication for food fraud control; detection of unauthorized GMOs in food products; molecular epidemiology investigations in cases of foodborne illness.
Technology facilitates the discovery of new pathogens (bacteria, viruses, and fungi) regardless of in vitro culture and knowledge of genotypic or phenotypic characteristics; comprehensive genome characterization of specific microorganisms, whether isolated or from a mixed sample; high-throughput screening, useful for epidemiological investigations.
This cutting-edge technology, thanks to its more sensitive and broad-spectrum analysis capabilities, allows simultaneous investigation of a considerable number and types of DNA polymorphic regions for individual identification, inference of phenotypic characteristics (iris color, hair color, skin complexion, height, age, predisposition to baldness, and facial morphology), and geographic origin. Furthermore, in cases where the sample is degraded, the analysis of the complete mitochondrial genome provides useful information for individual identification.
OUR TEAM
Roberto Cusano
Biotechnologist
– Head of Next Lab –
Gessica Milia
Biologist
– Researcher –
Rossano Atzeni
Bioinformatician
– Researcher –
Lidia Leoni
Director of Biosciences and Computational Infrastructures, Smart Projects and Quantum Computing
Last Publications
Littera Roberto, Cusano Roberto, Giglio Sabrina
Manca Maria Antonietta, Cusano Roberto, Sechi Leonardo
Dattilo Alessia, Ceccarini Giovanni, Scabia Gaia, Magno Silvia, Quintino Lara, Pelosini Caterina, Salvetti Guido, Cusano Roberto, Massidda Matteo, Montanelli Lucia, Gilio Donatella, Gatti Gianluca, Giacomina Alessandro, Costa Mario, Santini Ferruccio, Maffei Margherita
Cherchi Roberto, Cusano Roberto, Ferrari Paolo Albino, Massidda Matteo, Fotia Giorgio, De Matteis Sara, Cocco Pierluigi
Serra Marina, Cusano Roberto, Kowalik Marta Anna
Grandi Nicole, Cusano Roberto, Rubino Salvatore
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