{"id":12680,"date":"2025-07-03T18:18:15","date_gmt":"2025-07-03T16:18:15","guid":{"rendered":"https:\/\/www.crs4.it\/projects\/spoke-6-rna-drug-development\/"},"modified":"2026-04-02T12:32:39","modified_gmt":"2026-04-02T10:32:39","slug":"spoke-6-rna-drug-development","status":"publish","type":"projects","link":"https:\/\/www.crs4.it\/en\/projects\/spoke-6-rna-drug-development\/","title":{"rendered":"SPOKE 6 RNA DRUG DEVELOPMENT"},"content":{"rendered":"<p>The project is carried out in collaboration with the Spoke 6 (CNR IGM &#8211; Istituto di Genetica Molecolare &#8220;Cavalli-Sforza&#8221; Pavia) of the\u00a0National Center for Gene Therapy and Drugs based on RNA Technology and integrates the clinical trial activities identified by the Spoke 6 with the laboratory and bioinformatics expertise present at CRS4.<br \/>\n\u00a0<br \/>\nThe experimental approach focuses on Next Generation Sequencing technology and data analysis.    The project is divided into 3 Work Packages: &#8211; WP1 activities will be aimed at the development of laboratory protocols and bioinformatics pipelines to be able to respond to all the needs of genetic-molecular characterization of the transcriptome and genome; &#8211; WP2 involves the validation of the workflow including all protocols and pipelines developed by performing replicate experiments on samples with known results; &#8211; WP3 will consist of the application of the workflow on samples that will be sent to the CRS4 NGS platform with a flow of 6 patients per month.<br \/>\n\u00a0<br \/>\nProtocols based on NGS technology including structural changes (short read technology) and post-transcriptional chemical modifications (native RNA\/DNA long read technology) of coding and non-coding RNAs will be developed for comprehensive expression profile analysis.<br \/>\n\u00a0<br \/>\nGenome sequencing will provide useful information to elucidate the origins, genetic and epigenetic, of neoplastic transformation of cells. The data produced will be uploaded to an analysis and sharing platform called EvaDB.<\/p>\n","protected":false},"featured_media":11656,"template":"","meta":{"_acf_changed":false},"gruppo":[],"class_list":["post-12680","projects","type-projects","status-publish","has-post-thumbnail","hentry"],"acf":{"title":"SPOKE 6 RNA DRUG DEVELOPMENT","acronym":"SPOKE 6 RNA DRUG DEVELOPMENT","project_uuid":"a5d40867-0c58-11ef-8ceb-506b8da9258c","start_date":"01\/06\/2024","end_date":"30\/11\/2025","funder":"MUR  PNRR M4C2 - Investimento 1.4 -  Avviso Centri Nazionali - Centro nazionale per la terapia genica e i farmaci basati sulla tecnologia a RNA","partners":"\n<table style=\"width:100%; border-collapse: collapse;\">\n  <tr>\n    <th style=\"border: 1px solid #ddd; padding: 8px; background-color: #f2f2f2;\">Nome<\/th>\n    <th style=\"border: 1px solid #ddd; padding: 8px; background-color: #f2f2f2;\">Nazione<\/th>\n    <th style=\"border: 1px solid #ddd; padding: 8px; background-color: #f2f2f2;\">Ruolo<\/th>\n  <\/tr>\n\n  <tr>\n    <td style=\"border: 1px solid #ddd; padding: 8px;\"><a href=\"https:\/\/www.cnr.it\/\" target=\"_blank\">Consiglio Nazionale delle Ricerche<\/a><\/td>\n    <td style=\"border: 1px solid #ddd; padding: 8px;\">Italy<\/td>\n    <td style=\"border: 1px solid #ddd; padding: 8px;\">Coordinator<\/td>\n  <\/tr>\n<\/table>","proj_website":"https:\/\/www.rna-genetherapy.eu\/","project_status":"Negotiation","project_coordinator":[{"ID":10257,"post_author":"4","post_date":"2025-06-30 17:23:42","post_date_gmt":"2025-06-30 15:23:42","post_content":"Lidia Leoni is the High Performance and Quantum Computing, Biosciences and Advanced Studies director at the Center for Advanced Studies, Research, and Development in Sardinia (CRS4). She won a study grant from the European Economic Community and successfully attended a three-year course in information sciences and remote sensing. She is responsible for some of the most important CRS4 research and innovation projects. Her experience ranges from the use of large systems, to application development, to the management and maintenance of networks, systems and services for users. As a sector director, she is involved in several CRS4 projects and contributes to helping other researchers in their work. She is responsible for the biosciences and computational infrastructures of the center , for project services and manages the budget for the purchases of the computing center. Before joining CRS4, she worked at the Sardinia Research Consortium (CORISA), Regional Agrometeorological Service for Sardinia (SAR) and was a consultant for various institutions and companies.","post_title":"Lidia Leoni","post_excerpt":"","post_status":"publish","comment_status":"closed","ping_status":"closed","post_password":"","post_name":"lidia-leoni","to_ping":"","pinged":"","post_modified":"2026-04-05 20:11:50","post_modified_gmt":"2026-04-05 18:11:50","post_content_filtered":"","post_parent":0,"guid":"https:\/\/www.crs4.it\/people\/lidia-leoni\/","menu_order":0,"post_type":"people","post_mime_type":"","comment_count":"0","filter":"raw"}],"team_members":[{"ID":10137,"post_author":"4","post_date":"2025-06-30 17:22:02","post_date_gmt":"2025-06-30 15:22:02","post_content":"Riccardo Berutti, responsible of NGS Bioinformatics @CRS4, works since 2009 in the next generation sequencing field. He developed some among the first NGS analysis pipelines and quality control tools. Riccardo started his career in the Sardinian Genome Study stemming from the ProgeNIA project, on which he worked for his PhD thesis. His results were published with the collaboration on the journal Science.  In 2013 he moved to the Helmholtz Center in Munich for his postdoc with Prof. Thomas Meitinger and Dr. Tim Strom and continued at the Institute of Human Genetics (IHG) of the University Hospital of the Technical University of Munich (TUM), where he was head of the Bioinformatics group till December 2023 and so far produced around 60 peer reviewed publications. At the IHG he managed NGS data analysis applications for research and diagnostics. In the clinical field he acquired a strong experience as software developer, in process control, and in quality management, contributing to the ISO15189 certification of the Institute as a diagnostic laboratory, covering laboratory processes and the bioinformatic analyses developed in house. He is responsible of the development of the open source software EVAdb, for the clinical interpretation of the variants from human genomes and exomes and RNAseq. He also manage the development of their associated pipelines and of the EVAdb LIMS. He will continue the development of these open source tools in a collaborative form at CRS4.","post_title":"Riccardo Berutti","post_excerpt":"","post_status":"publish","comment_status":"closed","ping_status":"closed","post_password":"","post_name":"riccardo-berutti","to_ping":"","pinged":"","post_modified":"2026-04-05 20:10:08","post_modified_gmt":"2026-04-05 18:10:08","post_content_filtered":"","post_parent":0,"guid":"https:\/\/www.crs4.it\/people\/riccardo-berutti\/","menu_order":0,"post_type":"people","post_mime_type":"","comment_count":"0","filter":"raw"},{"ID":10206,"post_author":"4","post_date":"2025-06-30 17:22:41","post_date_gmt":"2025-06-30 15:22:41","post_content":"Roberto Cusano, responsible of NGS sequencing platform, works at the CRS4 since 2009 where he has specialized inNext Generation Sequencing. After graduating in Biotechnology at the University of Genoa, he worked in various public research centers and hospital institutions in Italy where he acquired vast experience (30 years) in biotechnology applied to research and diagnostics in the fields of molecular biology, rare diseases, oncology and transplantation genetics. Since his early working years he has specialized in techniques for manipulating and analyzing nucleic acids, in particular for genetic mapping and sequencing. The acquired skills are used both in the development of methods applied to diagnostics and research, and in providing scientific support and services to the scientific community. He is the author of 78 publications in international scientific journals subject to editorial control and with an H-index of 35","post_title":"Roberto Cusano","post_excerpt":"","post_status":"publish","comment_status":"closed","ping_status":"closed","post_password":"","post_name":"roberto-cusano","to_ping":"","pinged":"","post_modified":"2026-04-05 20:10:52","post_modified_gmt":"2026-04-05 18:10:52","post_content_filtered":"","post_parent":0,"guid":"https:\/\/www.crs4.it\/people\/roberto-cusano\/","menu_order":0,"post_type":"people","post_mime_type":"","comment_count":"0","filter":"raw"}]},"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v25.9 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>SPOKE 6 RNA DRUG DEVELOPMENT - CRS4<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.crs4.it\/en\/projects\/spoke-6-rna-drug-development\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"SPOKE 6 RNA DRUG DEVELOPMENT - CRS4\" \/>\n<meta property=\"og:description\" content=\"The project is carried out in collaboration with the Spoke 6 (CNR IGM &#8211; 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