{"id":10206,"date":"2025-06-30T17:22:41","date_gmt":"2025-06-30T15:22:41","guid":{"rendered":"https:\/\/www.crs4.it\/people\/roberto-cusano\/"},"modified":"2026-04-05T20:10:52","modified_gmt":"2026-04-05T18:10:52","slug":"roberto-cusano","status":"publish","type":"people","link":"https:\/\/www.crs4.it\/en\/people\/roberto-cusano\/","title":{"rendered":"Roberto Cusano"},"content":{"rendered":"<p>Roberto Cusano, responsible of NGS sequencing platform, works at the CRS4 since 2009 where he has specialized inNext Generation Sequencing. After graduating in Biotechnology at the University of Genoa, he worked in various public research centers and hospital institutions in Italy where he acquired vast experience (30 years) in biotechnology applied to research and diagnostics in the fields of molecular biology, rare diseases, oncology and transplantation genetics. Since his early working years he has specialized in techniques for manipulating and analyzing nucleic acids, in particular for genetic mapping and sequencing. The acquired skills are used both in the development of methods applied to diagnostics and research, and in providing scientific support and services to the scientific community. He is the author of 78 publications in international scientific journals subject to editorial control and with an H-index of 35<\/p>\n","protected":false},"featured_media":4183,"template":"","meta":{"_acf_changed":false},"gruppo":[124,125],"class_list":["post-10206","people","type-people","status-publish","has-post-thumbnail","hentry","gruppo-high-performance-and-quantum-computing-biosciences-and-advanced-studies","gruppo-next-generation-sequencing-core-en"],"acf":{"name":"Roberto","surname":"Cusano","email":"robycuso@crs4.it","phone":"","user_uuid":"e3c3da50-99fc-1032-95fe-0030485a3848","personal_page":"","google_scholar_id":"","position":"Technologist","project_coordinator":"","microsoft_academic_id":"","team_members":[{"ID":12560,"post_author":"4","post_date":"2025-07-03 18:17:42","post_date_gmt":"2025-07-03 16:17:42","post_content":"H2UB is intended to be a Life Science infrastructure aimed at fostering new drug development through a network of laboratories capable of providing basic, translational and preclinical research support using cutting-edge technologies.\nGovernance of the Hub will be centralized and will provide support in regulatory, ethics and management, as well as organizational and dissemination.   Expertise will be articulated in thematic platforms that will include:\n<ul>\n<li>basic research (biobanks of iPS and organoids as models of pathology; identification of specific molecular targets; fabrication of bio-electronic systems for drug evaluation);\u00a0<\/li>\n<li>pre-clinical research (animal models, identification and validation of disease biomarkers through computational data analysis, pharmacodynamics);<\/li>\n<li>new drug development (manufacturing, safety monitoring, specificity and formulation);<\/li>\n<li>clinical consulting.\u00a0<\/li>\n<\/ul>\nH2UB meets the need to manage and develop research infrastructure of high complexity. Its value is measured not only in the list of resources and services made available but also as a space where researchers can be stimulated to improve the way they do research by providing expertise, technology, and managerial support.\n\u00a0\nCRS4 will contribute to the:\n<ul>\n<li>implementation and validation of innovative technology platforms for Drug Screening through the definition of genomic and epigenomic targets to be evaluated and the validation of results using genomic techniques;<\/li>\n<li>study, development and implementation of flexible and scalable tools for computational pathology;<\/li>\n<li>developing of molecular modeling tools, to be implemented in the computing platform, with reference to the druggability of molecules and potential drugs on specific therapeutic targets;<\/li>\n<li>organization of training activities and schools on molecular modeling and metabolomics.<\/li>\n<\/ul>","post_title":"H2UB","post_excerpt":"","post_status":"publish","comment_status":"closed","ping_status":"closed","post_password":"","post_name":"h2ub","to_ping":"","pinged":"","post_modified":"2026-04-02 12:31:59","post_modified_gmt":"2026-04-02 10:31:59","post_content_filtered":"","post_parent":0,"guid":"https:\/\/www.crs4.it\/projects\/h2ub\/","menu_order":0,"post_type":"projects","post_mime_type":"","comment_count":"0","filter":"raw"},{"ID":12680,"post_author":"4","post_date":"2025-07-03 18:18:15","post_date_gmt":"2025-07-03 16:18:15","post_content":"The project is carried out in collaboration with the Spoke 6 (CNR IGM - Istituto di Genetica Molecolare \"Cavalli-Sforza\" Pavia) of the\u00a0National Center for Gene Therapy and Drugs based on RNA Technology and integrates the clinical trial activities identified by the Spoke 6 with the laboratory and bioinformatics expertise present at CRS4.\n\u00a0\nThe experimental approach focuses on Next Generation Sequencing technology and data analysis.    The project is divided into 3 Work Packages: - WP1 activities will be aimed at the development of laboratory protocols and bioinformatics pipelines to be able to respond to all the needs of genetic-molecular characterization of the transcriptome and genome; - WP2 involves the validation of the workflow including all protocols and pipelines developed by performing replicate experiments on samples with known results; - WP3 will consist of the application of the workflow on samples that will be sent to the CRS4 NGS platform with a flow of 6 patients per month.\n\u00a0\nProtocols based on NGS technology including structural changes (short read technology) and post-transcriptional chemical modifications (native RNA\/DNA long read technology) of coding and non-coding RNAs will be developed for comprehensive expression profile analysis.\n\u00a0\nGenome sequencing will provide useful information to elucidate the origins, genetic and epigenetic, of neoplastic transformation of cells. The data produced will be uploaded to an analysis and sharing platform called EvaDB.","post_title":"SPOKE 6 RNA DRUG DEVELOPMENT","post_excerpt":"","post_status":"publish","comment_status":"closed","ping_status":"closed","post_password":"","post_name":"spoke-6-rna-drug-development","to_ping":"","pinged":"","post_modified":"2026-04-02 12:32:39","post_modified_gmt":"2026-04-02 10:32:39","post_content_filtered":"","post_parent":0,"guid":"https:\/\/www.crs4.it\/projects\/spoke-6-rna-drug-development\/","menu_order":0,"post_type":"projects","post_mime_type":"","comment_count":"0","filter":"raw"},{"ID":12706,"post_author":"4","post_date":"2025-07-03 18:18:45","post_date_gmt":"2025-07-03 16:18:45","post_content":"The ARRDIA project, articulated in 3 different tranches from 2020 to 2025,\u00a0concerns the study of early tumour markers in workers and population of particular industrial areas of Sardinia, with particular reference to pleuropulmonary pathologies resulting from asbestos dust of industrial nature.\n\u00a0\nThe project aims to develop innovative non-invasive methods to detect and monitor the tumours under study. In particular, the research aims at detecting MicroRNAs in the breath and plasma of ex-exposed people and comparison groups, in order to be able to intervene at an early stage and treat possible pathologies in a timely manner.\n\u00a0\nFurther information and details can be found at the project Web site <a>https:\/\/www.arrdia.it<\/a>","post_title":"ARRDIA","post_excerpt":"","post_status":"publish","comment_status":"closed","ping_status":"closed","post_password":"","post_name":"arrdia","to_ping":"","pinged":"","post_modified":"2026-04-02 12:32:52","post_modified_gmt":"2026-04-02 10:32:52","post_content_filtered":"","post_parent":0,"guid":"https:\/\/www.crs4.it\/projects\/arrdia\/","menu_order":0,"post_type":"projects","post_mime_type":"","comment_count":"0","filter":"raw"},{"ID":14965,"post_author":"4","post_date":"2025-07-03 18:19:37","post_date_gmt":"2025-07-03 16:19:37","post_content":"The project aims to consolidate and develop integrated and interoperable technologies for digital medicine able to support complex diagnostic and\/or experimental processes, integrating heterogeneous data from different sources (e.g., clinical databases, genomic analysis pipelines, molecular biology systems), according to the main international standards and guidelines. In this context, particular efforts will be targeting digital pathology tools and techniques.","post_title":"PAM","post_excerpt":"","post_status":"publish","comment_status":"closed","ping_status":"closed","post_password":"","post_name":"pam","to_ping":"","pinged":"","post_modified":"2026-04-02 12:33:31","post_modified_gmt":"2026-04-02 10:33:31","post_content_filtered":"","post_parent":0,"guid":"https:\/\/www.crs4.it\/projects\/pam\/","menu_order":0,"post_type":"projects","post_mime_type":"","comment_count":"0","filter":"raw"},{"ID":14972,"post_author":"4","post_date":"2025-07-03 18:19:40","post_date_gmt":"2025-07-03 16:19:40","post_content":"PATH combines automation technology, experience in proteomic and genomic diagnostics and expertise in the field of clinical informatics, in relation to processes and management of large amounts of biomedical data, to define enabling technologies that allow to substantially redesign the work paths of pathological anatomy laboratories in order to improve their productivity, and the quality of diagnosis and prognosis.\n<br\/>The main aim of the PATH project is to define enabling technologies that allow to substantially redesign the \"productive\" process of pathological anatomy laboratories. The mechanisms on which it proposes to act concern: the automation of the handling and storage of samples, in order to create chains such as Total Laboratory Automation for the pipelines of analysis; the traceability of samples and the efficient remote access to the results in order to ensure an efficient interface of the laboratory with external hospital facilities.\n<br\/>The various prototypes and the research and development activities related to them will demonstrate in a concrete way the possibility of extending to the field of pathological anatomy the ICT and process automation technologies already widespread and consolidated in other diagnostic sectors, such as laboratory analysis and radiology, despite the substantial differences in the process and the different clinical needs.\nIn addition to demonstrating the feasibility of this evolution in the field of pathological anatomy, it will be highlighted, through studies and some specific implementations, how this paradigm shift can lay the foundations for the near future of clinical diagnostics, creating the conditions for the creation of an integrated diagnostic laboratory (clinical chemistry\/pathological anatomy) that includes in the diagnostic workflow innovative methods such as genomics and proteomics procedures, with a view to progress towards personalized medicine.\n<br\/>The objectives considered are at the frontier of the state of the art for industrial research: the results of the activities will be specialized in oncology, but they are of general interest and the innovative models of activities in the field of health can be adopted and extended in other clinical contexts, also thanks to the use of open and interoperable technological solutions.\nThe training project, associated with the PATH research project, aims to train industrial researchers with specific skills in the most innovative aspects of modern digital medicine technologies such as: research and development of innovative systems of robotized management of samples for complex examinations on multiple markers and different diagnostic methods; techniques for integration of digital services for diagnostics with new experimental methods (high-processor sequencing, proteomics, genetic profiling); scalable methodologies of management, transfer, analysis and visualization of large moles of biomedical data.","post_title":"PATH","post_excerpt":"","post_status":"publish","comment_status":"closed","ping_status":"closed","post_password":"","post_name":"path","to_ping":"","pinged":"","post_modified":"2026-04-02 12:33:34","post_modified_gmt":"2026-04-02 10:33:34","post_content_filtered":"","post_parent":0,"guid":"https:\/\/www.crs4.it\/projects\/path\/","menu_order":0,"post_type":"projects","post_mime_type":"","comment_count":"0","filter":"raw"},{"ID":14990,"post_author":"4","post_date":"2025-07-03 18:19:55","post_date_gmt":"2025-07-03 16:19:55","post_content":"The project aims to characterise the epidemiological and phylogenetic  dynamics regarding the current distribution and spread of SARS-CoV-2  respiratory infection on the regional territory, including cases  detected in the reference hospitals of the main urban areas (Cagliari,  Nuoro and Sassari).","post_title":"CarGen4CoV","post_excerpt":"","post_status":"publish","comment_status":"closed","ping_status":"closed","post_password":"","post_name":"cargen4cov","to_ping":"","pinged":"","post_modified":"2026-04-02 12:33:48","post_modified_gmt":"2026-04-02 10:33:48","post_content_filtered":"","post_parent":0,"guid":"https:\/\/www.crs4.it\/projects\/cargen4cov\/","menu_order":0,"post_type":"projects","post_mime_type":"","comment_count":"0","filter":"raw"},{"ID":14992,"post_author":"4","post_date":"2025-07-03 18:19:57","post_date_gmt":"2025-07-03 16:19:57","post_content":"Epitopes are those small parts of pathogens that antibodies use to bind  to them and destroy them. The aim is to identify by computational  methods the specific epitopes of the two coronaviruses NL63 and CoV-2  and homologous epitopes in common. Among the objectives are to  synthesise the specific epitopes for testing in sera from patients with  autoimmune, inflammatory and neurodegenerative diseases, but also to  understand the reasons for the different susceptibility to SARS-CoV-2  infection, including with respect to symptom severity and response to  treatment.","post_title":"Epitopes4cov","post_excerpt":"","post_status":"publish","comment_status":"closed","ping_status":"closed","post_password":"","post_name":"epitopes4cov","to_ping":"","pinged":"","post_modified":"2026-04-02 12:33:49","post_modified_gmt":"2026-04-02 10:33:49","post_content_filtered":"","post_parent":0,"guid":"https:\/\/www.crs4.it\/projects\/epitopes4cov\/","menu_order":0,"post_type":"projects","post_mime_type":"","comment_count":"0","filter":"raw"},{"ID":14994,"post_author":"4","post_date":"2025-07-03 18:19:58","post_date_gmt":"2025-07-03 16:19:58","post_content":"The aim of the project is to develop a portable electronic system for  analysing SARS-CoV-2 viral RNA, which can be applied to easy-to-obtain  biological matrices such as saliva, and which provides a sufficiently  precise screening result, to be subsequently confirmed by swab analysis.","post_title":"RNA4CoV","post_excerpt":"","post_status":"publish","comment_status":"closed","ping_status":"closed","post_password":"","post_name":"rna4cov","to_ping":"","pinged":"","post_modified":"2026-04-02 12:33:51","post_modified_gmt":"2026-04-02 10:33:51","post_content_filtered":"","post_parent":0,"guid":"https:\/\/www.crs4.it\/projects\/rna4cov\/","menu_order":0,"post_type":"projects","post_mime_type":"","comment_count":"0","filter":"raw"},{"ID":14996,"post_author":"4","post_date":"2025-07-03 18:19:59","post_date_gmt":"2025-07-03 16:19:59","post_content":"The project idea stems from the healthcare system's need for rapid  laboratory methods for the diagnosis of CoV-2 infection, associated with  a shortage of commercial reagents and kits. The project aims to develop  a Next Generation Sequencing (NGS) analysis protocol able to detect the  presence of viral RNA in a large number of biological samples, in a  short time (24h), at reduced costs and with increased sensitivity.","post_title":"NGS4CoV","post_excerpt":"","post_status":"publish","comment_status":"closed","ping_status":"closed","post_password":"","post_name":"ngs4cov","to_ping":"","pinged":"","post_modified":"2026-04-02 12:33:52","post_modified_gmt":"2026-04-02 10:33:52","post_content_filtered":"","post_parent":0,"guid":"https:\/\/www.crs4.it\/projects\/ngs4cov\/","menu_order":0,"post_type":"projects","post_mime_type":"","comment_count":"0","filter":"raw"},{"ID":18994,"post_author":"4","post_date":"2025-07-03 18:20:19","post_date_gmt":"2025-07-03 16:20:19","post_content":"The project aims to develop a non-invasive protocol to analyze the genetic profile of ctDNA (circulating tumor DNA) extracted from liquid biopsy of haematological and oncological patients, through the application of next generation sequencing technologies (NGS) on a panel of genes.\u00a0\nNIASMIC, promoted by the regional agency Sardegna Ricerche through the \"Cluster top-down actions\" program, is financed through the POR FESR of the Autonomous Region of Sardinia 2014-2020","post_title":"NIASMIC","post_excerpt":"","post_status":"publish","comment_status":"closed","ping_status":"closed","post_password":"","post_name":"niasmic","to_ping":"","pinged":"","post_modified":"2026-04-02 12:34:08","post_modified_gmt":"2026-04-02 10:34:08","post_content_filtered":"","post_parent":0,"guid":"https:\/\/www.crs4.it\/projects\/niasmic\/","menu_order":0,"post_type":"projects","post_mime_type":"","comment_count":"0","filter":"raw"},{"ID":19607,"post_author":"4","post_date":"2025-07-03 18:21:12","post_date_gmt":"2025-07-03 16:21:12","post_content":"Genetic modifiers predisposing to CKD in Alport and thin basement membrane nephropathy","post_title":"COL 4","post_excerpt":"","post_status":"publish","comment_status":"closed","ping_status":"closed","post_password":"","post_name":"col-4","to_ping":"","pinged":"","post_modified":"2026-04-02 12:35:02","post_modified_gmt":"2026-04-02 10:35:02","post_content_filtered":"","post_parent":0,"guid":"https:\/\/www.crs4.it\/projects\/col-4\/","menu_order":0,"post_type":"projects","post_mime_type":"","comment_count":"0","filter":"raw"}]},"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v25.9 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Roberto Cusano - CRS4<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.crs4.it\/en\/people\/roberto-cusano\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Roberto Cusano - CRS4\" \/>\n<meta property=\"og:description\" content=\"Roberto Cusano, responsible of NGS sequencing platform, works at the CRS4 since 2009 where he has specialized inNext Generation Sequencing. 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