Roberto Cusano

Roberto Cusano

CURRICULUM VITAE

Name: Roberto Cusano

Date and place of birth: Genoa, 25 January 1970

Citizenship: Italian

Status: Married

Forein language: English

Education:

1991-1994: Graduated "cum Laude" at the University school for Technician in Biotechnology, University of Genoa.

1999-2000: Graduated "cum Laude" in University degree for Technician in Biotechnology, University of Genoa.

2002-2003: Bachelor degree in Biotechnology at the University of Genoa

Professional activities:

1994-1995: Collaborator at the Department of Biology and Genetics (IBIG) University of Genoa (Director Prof. F.Ajmar) Main activities: Linkage analysis in large families with recurrent hypertrophic cardiomyopathy; Mutation screening by SSCP and sequencing analysis; Production of lymphoblastoid cell lines.

1995-1997: Collaborator at the Laboratory Neuroblastoma Research of the Advanced Biotechnology Center of Genoa (Director Dr. G.P.Tonini). Main activities: Molecular diagnosis on tissue of NB patients; Linkage analysis in families with recurrent NB.

1997-2002: Collaborator at the Laboratory of Molecular Genetics, G.Gaslini Institute of Genoa (Coordinator Prof. M.Seri). Main activities: Sequencing and genotyping service manager; Linkage analysis by Cyrillic e MLINK software; Molecular characterization of critical regions and disease gene candidates by computational study of genetic databases; Mutation screening by DHPLC, SSCP and sequencing analysis; Human-Hamster hybrid cell production; Selection and preparation of YAC, BAC, Cosmid and Plasmid derived probes for FISH mapping and Southern Blot analysis.

2001: Collaborator at the Laboratory of Human Genetics, Galliera Hospital of Genoa (Director Dr. F. Dagna Bricarelli). Main activities: Knowledge transfer of sequencing techniques and use of analysis software; Strategy study and protocol setup for molecular diagnosis in genetic diseases; Molecular diagnosis of hereditary disease.

2001-2002: Collaborator at the Department of Oncology, Biology and Genetics (DOBiG), University of Genoa (Director Prof. G. Bianchi-Scarrà). Main activities: Setup and knowledge transfer in DHPLC and sequencing analysis techniques.

2002-2003: Collaborator at the Laboratory of Human Genetics, Sant'Orsola-Malpighi Hospital of Bologna (Director Prof. G. Romeo, Coordinator Prof. M.Seri). Main activities: Start up and organization of the lab, management of the material supply and evaluation of the instruments and equipments required; Analysis of genetic polymorphisms and gene sequences by ABI3730 Sequencer, GeneMapper Software 3.0 and Sequence Analysis Software 5.0.

2003-2004: Collaborator at the Laboratory of Molecular Genetics, G.Gaslini Institute of Genoa (Coordinator Dr. L.J. Galietta). Main activities: Cloning and mutagenesis of ion channel genes.

2003-2005: Collaborator at the Laboratory of Human Genetics, Galliera Hospital of Genoa (Director Dr. F. Dagna Bricarelli). Main activities: Molecular diagnosis of hereditary disease; Knowledge transfer of sequencing techniques and use of analysis software.

2004-2008: Collaborator at the Tissue Typing Laboratory of Italian Bone Marrow Donor Registry (IBMDR), Galliera Hospital of Genoa (Director Dr. Nicoletta Sacchi). Main activities: Sequencing based typing of HLA loci, analysis and reporting by Assign software; Development of strategies. protocols for genetic analysis of the HLA system.

2004-2008: Collaborator at the Department of Oncology, Biology and Genetics (DOBiG), University of Genoa (Director Prof. G. Bianchi-Scarrà). Main activities: Structural characterization of genes involved in hereditary disease and development of strategies for molecular analysis and diagnostic protocols; Analysis of transcribed sequences by RT-PCR, sequencing techniques and Real-time PCR; Molecular diagnosis of disease predisposing genes.

2006: Collaborator at the Laboratory of Translational Oncology, S.Croce-Carle Hospital of Cuneo (Coordinator Dr C. Lo Nigro). Main activities: Analysis of expression, methylation pattern, polymorphisms and somatic mutations of genes involved in the drug response variability in cancer patients.

2007-2008: Collaborator at the Muscular and Neurodegenerative Disease Unit, G. Gaslini Institute of Genoa (Coordinator Dr. F. Zara). Main activities: Apprentice education and knowledge transfer of molecular genetics techniques and strategies for high throughput analysis of candidate genes.

2009-2011: Collaborator at the Laboratory of Immunogenetics c/o Technology Park - Polaris of Pula (Director Prof. F. Cucca - University of Sassari and IRGB-CNR Cagliari). Main activities: Generation of sequence data by NGS instruments Illumina Genome Analyzer IIx and HiSeq2000; Sample preparation and processing for whole genome and whole transcriptome sequencing; Development of strategies and protocols for genetic analysis.

2012: Collaborator at the Sequencing and Genotyping Platform, CRS4 c/o Technology Park Polaris of Pula, Sardinia (Coordinator Dott. A. Angius). Main activities: Generation of sequence data by NGS instruments Illumina Genome HiSeq2000; Sample preparation and processing for DNA-Seq, RNA-Seq, ChIP-Seq, Exome-Seq, target resequencing experiments; evaluation and development of strategies for the analysis of nucleic acids using NGS; Sanger and Next-Generation Sequencing service for external people; Whole transcriptome expression studies by Affymetrix platform; Maintenance and troubleshooting of Illumina sequencers HiSeq2000 and Applied Biosystems 3730; Exome analysis using Galaxy platform and Linux OS programs.

Position

Consultant

Contacts

Email

robycuso@crs4.it

Phones

Fax

Google Scholar

e3c3da50-99fc-1032-95fe-0030485a3848

Publications


Projects


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